Essays about: "gene amplification"

Showing result 1 - 5 of 42 essays containing the words gene amplification.

  1. 1. Development of a qPCR method for detection and quantification of Ustilago nuda and COX1 gene in Barley seeds

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Umer Shaukat Ali; [2023]
    Keywords : ;

    Abstract : Barley loose smut caused by the fungal pathogen Ustilago nuda is a global concern with detrimental effects on barley production. Early detection of this infection is vital for effective disease supervision. However, current seed health testing protocols suffer from limitations in terms of time and efficiency. READ MORE

  3. 2. CRISPR Screen to Identify Genes Regulating Melanoma Cell Invasiveness

    University essay from KTH/Proteinvetenskap

    Author : Emma Kroon; [2023]
    Keywords : CRISPR-Cas9; gene knockout; viral packaging; transduction; invasionstest; CRISPR-Cas9; genknockout; viruspackning; transduction; invasion assay;

    Abstract : Maligna cancerceller har förmågan att sprida sig okontrollerat i kroppen och metastasera till avlägsna organ. Kritiskt i metastaserings processen är cancercellers förmåga att invadera närliggande vävnader innan de sprider sig vidare ut i kroppen. READ MORE

  4. 3. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Wilma Wadman; [2023]
    Keywords : BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

    Abstract : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. READ MORE

  5. 4. Quantification of DNA Nanoballs Using Image Processing Techniques

    University essay from Uppsala universitet/Avdelningen Vi3

    Author : Sara Lindberg; [2023]
    Keywords : image analysis; fluorescence microscopy; quantification; DNA; rolling circle amplification; machine learning; deep learning; transfer learning; neural networks;

    Abstract : In gene editing, it is important to identify the number of edited and unedited nucleic acids in the development of new therapies and drugs. Countagen is developing a technology for accelerating genomic research and their product is called GeneAbacus. READ MORE

  6. 5. Quantification of DNA Microballs Using Image Processing Techniques

    University essay from KTH/Industriell bioteknologi

    Author : Yosef Werede Tedros; [2023]
    Keywords : Spot detection; Deep learning; Image processing; smFISH; Gene editing; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot; Detektering av punkter; Djupinlärning; Bildbehandling; smFISH; Genredigering; CRISPR; Rolling circle amplification; Big-FISH; LodeSTAR; DeepSpot;

    Abstract : I detta examensarbete användes olika bildbehandlingstekniker för detektion och kvantifiering av DNA-mikrobollar, mer specifikt rolling circle amplification-produkter, på mikroskopibilder. Avsikten med detta arbete var att hjälpa Countagen AB utforska pipelines för bildbehandling för sin produkt där de analyserar utfallet av genredigeringsförsök på ett billigare och snabbare sätt än dagens konventionella sekvenseringsmetoder. READ MORE