Essays about: "multiplex qPCR"

Found 2 essays containing the words multiplex qPCR.

  1. 1. Development and evaluation of procedures and reagents for extraction of proteins from dried blood spots for analysis using Proseek

    University essay from Institutionen för biologisk grundutbildning

    Author : Johan Björkesten; [2014]
    Keywords : DBS; dried blood spots; PEA; Proseek; Proseek Multiplex; qPCR; Protein detection; Olink;

    Abstract : A method for extraction of proteins from dried blood spots (DBS) for analysis using Proseek is developed and evaluated. DBS, as sample format, possesses a number of desirable advantages over for example plasma samples. These advantages include for example minimal patient invasiveness, sampling simplicity and non regulated sample transportation. READ MORE

  2. 2. Optimization of the multiplexed Proximity Ligation Assay for detection of blood-based biomarkers

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Martin Lundberg; [2014]
    Keywords : PLA; colorectal cancer; biomarker; multiplex qPCR; Olink;

    Abstract : The Proximity Ligation Assay (PLA) is a relatively new method which utilizes the strength of both immunoassays and DNA detection. PLA has the capacity of high multiplexing due to the high specificity achieved with both dual protein-binding and dual primer binding during detection with Real-Time PCR. READ MORE