Essays about: "Immobilized enzyme"

Showing result 1 - 5 of 11 essays containing the words Immobilized enzyme.

1. 1. Production, in vitro modification, and interaction analysis of a hydroxyproline-dependent protein

   University essay from Uppsala universitet/Biokemi

   Author : Milica Plavsic; [2023]
   Keywords : protein production optimisation; recombinant expression; bioreactor; prolyl hydroxylase; liquid chromatography mass spectrometry; microscale thermophoresis;

   Abstract : The development of a biologic protein involves different stages and becomes a highly complex process which can be costly and time consuming to scale up for industrial production. Therefore, optimization is a necessary part of the production process development to lower the production expenses. READ MORE

3. 2. BIOMIMETIC SENSORS FOR RAPID AND SENSITIVE SARS-CoV-2 DETECTION

   University essay from Malmö universitet/Fakulteten för hälsa och samhälle (HS)

   Author : Maryam Hmouda; [2022]
   Keywords : ;

   Abstract : In the last two years, the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted the entire world. SARS-CoV-2 detection methods include Polymerase Chain Reaction (PCR), which is expensive, and Rapid Antigen Test, which is not highly sensitive. READ MORE

4. 3. Biosensor based on immobilized amine transaminase for detection of amphetamine

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Clara Öh; [2020]
   Keywords : amine transaminases ATA ; enzyme immobilization; biosensor; covalent immobilization; ionic immobilization; amphetamine; histidine-tagged enzyme; Poly methyl methacrylate PMMA ;

   Abstract : Amine transaminases (ATA) catalyse the transfer of an amino group from one molecule and replaces a ketone or aldehyde with the amino group, the amino group on the amino-donor is replaced with a ketone or aldehyde. This enzyme, ATA from Chromobacterium violaceum, has previously been used to catalyse the reaction involving amphetamine, therefore, it might be possible to use this enzyme to convert amphetamine and the product absorbs in the UV spectrum and can therefore be measured spectrophotometrically. READ MORE

5. 4. Development of a Water Activity Control and Reaction Monitoring System for Acidolysis and Transesterification Reactions using Immobilized Lipases in a Rotating Bed Reactor

   University essay from Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

   Author : Eimantas Gladkauskas; [2019]
   Keywords : Enzyme; Enzymes; Immobilized enzyme; bioreactor; rotating bed reactor; acidolysis; transesterification; arduino; water activity; thermodynamic water activity; Enzyme activity; automatic reaction tracking; reaction tracking; biotechnology; electrical engineering; relative humidity; spinchem; enzymatic synthesis; water activity control; process automation; lipases; lipase; bioteknik; Technology and Engineering;

   Abstract : Controlled water activity in an enzymatic reaction can influence enzyme activity and productivity. A small layer of water around an enzyme gives it a required flexibility to catalyze reactions at a higher rate in contrast with the one that is dry (Adlercreutz, Dicko, Larsson, & Månsson, 2017). READ MORE

6. 5. Biochemical characterization of resurrected ancestral ammonia lyases

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Albin Holmberg Larsson; [2019]
   Keywords : Ancestral sequence resurrection; Inborn errors of metabolism; Aromatic ammonia lyases; Enzyme kinetics; Thermostability;

   Abstract : This study set out to express, purify and characterize twelve ammonia lyase enzymes for potential application as a supplement to a treatment of an inborn error of metabolism disease. The DNA sequence for two wild-type ammonia lyases, three modified ammonia lyases and seven resurrected ancestral ammonia lyases had been synthesized and cloned in vectors. READ MORE