Essays about: "chinese hamster ovary"

Showing result 1 - 5 of 8 essays containing the words chinese hamster ovary.

1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

   University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Author : Basel Yousef; [2024]
   Keywords : ;

   Abstract : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. READ MORE

3. 2. Development of specific host cell protein assays

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Anna Ivert Nordén; [2023]
   Keywords : Gyrolab; Immunoassay; HCP;

   Abstract : The manufacturing and the development of biotherapeutic drugs involves the expression of biotherapeutic proteins in a host cell expression system followed by a purification process. Bioanalytical methods to measure impurities such as host cell proteins (HCPs) are needed to obtain a robust process and a safe drug according to regulatory requirements. READ MORE

4. 3. The roles of heparan sulfate in autophagy

   University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Author : Asifa Khan; [2022]
   Keywords : Heparan sulfate; LC3-II; p62; Autophagy;

   Abstract : Background: Autophagy is a lysosomal degradation process to eliminate damaged components from the cells. It also provides a survival mechanism in stressed conditions and limits growth processes. Heparan sulfate (HS) is a polysaccharide attached to different proteoglycans. READ MORE

5. 4. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

   University essay from Lunds universitet/Teknisk mikrobiologi

   Author : Eva Sjöblom; [2020]
   Keywords : BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

   Abstract : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. READ MORE

6. 5. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

   University essay from Linköpings universitet/Teknisk biologi

   Author : Malin Strannermyr; [2018]
   Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

   Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE