Essays about: "plasmids"

Showing result 16 - 20 of 50 essays containing the word plasmids.

  1. 16. Like a Rolling Circle : Developing in-situ genotyping of chromosomal barcodes in the DuMPLING method

    University essay from Uppsala universitet/Molekylär systembiologi

    Author : Fabian Svahn; [2021]
    Keywords : Rolling circle amplification; genotyping; chromosomal barcodes; RCA; DuMPLING method; DuMPLING; barcoded libraries; microfluidics;

    Abstract : DuMPLING is a newly developed high-throughput method to study singlecellphenotypes in a pooled and barcoded library using a microfluidicchip. The chip enables parallel biophysical measurements of singlecells, after which in-situ genotyping connects the cells to a certainstrain of the library. READ MORE

  3. 17. Engineering Rhodosporidium toruloides for the production of polyhydroxybutyrate

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Tova Lindh; [2021]
    Keywords : Applied microbiology; Bioplastics; Rhodosporidium toruloides; Polyhydroxybutyrate; Non-conventional yeast; Oleaginous yeast; Biology and Life Sciences;

    Abstract : The increased use of plastics has become an environmental problem where plastics, due to the slow degradation, accumulate in the oceans and landfills. Although plastics are a very cheap and useful material with desirable properties and a wide variety of applications, they are primarily made from petroleum-based resources which are non-renewable and of fossil origin. READ MORE

  4. 18. Construction and evaluation of a bacterial fluorescent conjugation tracking system

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Otto Lindahl; [2021]
    Keywords : ;

    Abstract : Plasmids are small, circular, extrachromosomal genetic elements that replicate independently of the bacterial chromosome. Plasmids play a crucial role in the dissemination of antibiotic resistance genes through conjugation. READ MORE

  5. 19. The transfer of chromosomal genes through bacterial conjugation in Escherichia coli

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Arijana Katana; [2021]
    Keywords : conjugation; escherichia coli; hybrid;

    Abstract : Evolution in bacteria occurs through the combined effects of spontaneous mutations and horizontal gene transfer (HGT). Several mechanisms can lead to HGT: (i) transformation, the uptake of DNA from the environment; (ii) transduction, the transfer of DNA carried by a bacteriophage into another bacterium during infection; and (iii) conjugation, bacterial mating mediated by a conjugative plasmid like the F-factor. READ MORE

  6. 20. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Farhana Manzoor; [2020]
    Keywords : Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

    Abstract : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. READ MORE