Essays about: "plasmids"

Showing result 11 - 15 of 50 essays containing the word plasmids.

  1. 11. Assessment of promoter regulation in Pseudomonas putida using GFP and flow cytometry

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Luka Cululejevic; [2022]
    Keywords : applied microbiology; Pseudomonas putida; promoter characterization; aromatic compounds; vanillin; guaiacol; Biology and Life Sciences;

    Abstract : Lignin is found in all biomass and is an abundant natural source of aromatic compounds. It has the potential to serve as a renewable raw material for a variety of chemicals currently derived from fossil fuel sources such as muconic acid, an important platform chemical. Kraft lignin is a waste stream from the pulp and paper industry. READ MORE

  3. 12. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

    University essay from Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Author : Aakash Reddy Murugadasan; [2022]
    Keywords : Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

    Abstract : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. READ MORE

  4. 13. The use of Gibson Assembly for DNA cloning

    University essay from KTH/Tillämpad fysik

    Author : Samuel Johansson; [2022]
    Keywords : Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

    Abstract : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. READ MORE

  5. 14. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Olivia Alexandra; [2021]
    Keywords : Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

    Abstract : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. READ MORE

  6. 15. Advanced Detection Methods of Genomic Barcodes for Genotyping Escherichia coli Libraries

    University essay from Uppsala universitet/Institutionen för cell- och molekylärbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Nicole Eger; [2021]
    Keywords : Genotyping; Genomic barcodes; LAMP; PNA; RCA; FISH;

    Abstract : Pooled cell strain libraries are a powerful tool allowing to investigate the influence of genetic modifications on phenotypes in high throughput single-cell assays. To link the genotype to phenotype in each cell of the library, unique 20 base pairs (bp) long barcodes are used to allow in situ genotyping after phenotyping via fluorescence microscopy. READ MORE