Essays about: "polyclonal antibodies"

Showing result 1 - 5 of 10 essays containing the words polyclonal antibodies.

  1. 1. VISUALIZING CATESTATIN AND CHROMOGRANIN A : An Antibody Validation

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Johan William-Olsson; [2022]
    Keywords : Diabetes; immunology; western blot; flow cytometry; immunofluorescence;

    Abstract : Chromogranin A (CgA) and its cleavage product Catestatin (CST) are two very interesting proteins, especially due to their possible involvement in the development of type 1 diabetes. There are currently only a few primary antibodies against CgA and CST, and the ones that do exist are often only recommended for use on one method. READ MORE

  3. 2. Evaluation of the immunogenicity of SARS-CoV-2 B cell epitopes

    University essay from Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Author : Sofia Hogander; [2022]
    Keywords : SARS-CoV-2; peptide vaccine; B cell epitope; ELISA; solid phase peptide synthesis;

    Abstract : Background: The COVID-19 pandemic is caused by the SARS-CoV-2 virus, which enter the host cells through interactions between the receptor-binding domain (RBD) on the S-protein and the ACE-2 receptor on the host cell. A novel type of vaccine strategy is peptide vaccines, with great potential as a faster and more selective approach to conventional vaccine development. READ MORE

  4. 3. Effects of antibody labeling chemistry on assays developed for the Gyrolab immunoassay platform

    University essay from Uppsala universitet/Molekylär systembiologi

    Author : Julia Dencker; [2021]
    Keywords : Antibodies; Labeling chemistry; Site-specific labeling; Immunoassay; Gyrolab;

    Abstract : The aim of this project was to make a comparison of the effects of antibody labeling chemistries on assays developed for the Gyrolab immunoassay platform. One of the labeling techniques was a heterogenous labeling technique targeting amino groups on the antibody. READ MORE

  5. 4. Mitigating the impact of antidrug antibodies against insulin on ELISA assay

    University essay from Malmö universitet/Institutionen för biomedicinsk vetenskap (BMV)

    Author : Thea Bøwadt; [2021]
    Keywords : Enzyme-Linked Immunosorbent Assay; ELISA; Antidrug antibodies; ADA; Insulin; Polyethylene glycol; PEG; Acid dissociation; MgCl2.;

    Abstract : Diabetes has, in the past three decades, surged immensely. Because of this, new insulin analogues are constantly in the making.  In clinical studies, the presence of antidrug antibodies can prove a challenge when measuring insulin. READ MORE

  6. 5. Development of a high-throughput platform for generation and early screening of high producing stable cell lines

    University essay from KTH/Proteinvetenskap

    Author : Jonathan Karlsson Persson; [2021]
    Keywords : CHO-K1; HEK293; CellCelector™; High-throughput screening; Protein A beads; Secondary antibody; CHO-K1; HEK293; CellCelector™; Högkapacitets-screening; Protein A-kulor; Sekundär antikropp;

    Abstract : Produktionen av rekombinanta biofarmaceutiska läkemedel, exempelvis monoklonala antikroppar, är en oumbärlig men ansträngande process. Under 2019 var sju av de tio mest sålda läkemedlen globalt baserade på rekombinant producerade monoklonala antikroppar. READ MORE