Essays about: "protein purification"

Showing result 6 - 10 of 111 essays containing the words protein purification.

  1. 6. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elnaz Samadian Zad; [2023]
    Keywords : adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Abstract : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. READ MORE

  3. 7. Optimization of two high-performance size-exclusion chromatography methods with diode-array detection for protein quantification and purity assessment

    University essay from Lunds universitet/Centrum för analys och syntes

    Author : Camilla Lane; [2023]
    Keywords : Method qualification; Protein concentration; Protein Purity; High Performance Size exclusion chromatography; Quality control; Analytical chemistry; Biology and Life Sciences;

    Abstract : Background: There is a need to develop methods of determining concentration and purity for two new affinity ligands, protein T and protein H, in order to ensure the quality of an industrial production of said proteins. Aims: To develop a High Performance Size Exclusion Chromatography, HPSEC, method to determine concentration and purity of protein T and to improve an existing HPSEC method for concentration and purity determination of protein H that show selectivity, display linearity between peak area and injection amount and give repeatable results. READ MORE

  4. 8. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Anna Králová; [2023]
    Keywords : time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Abstract : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. READ MORE

  5. 9. Water purification of ibuprofen using whey protein aerogels with and without PNF-structure

    University essay from KTH/Skolan för industriell teknik och management (ITM)

    Author : Jessica Lyrner Morén; David Jonsson; [2023]
    Keywords : Aerogels; water purification; ibuprofen; protein nanofibrils PNF ; whey protein; attitudes; scientific research; Aerogeler; vattenrening; ibuprofen; protein nanofibriller PNF ; vassleprotein; attityder; naturvetenskaplig forskning;

    Abstract : Clean water, free from pharmaceuticals, is important both for land and water living animals. Protein nanofibrils (PNFs) have already been shown to be a promising candidate for water purification of pharmaceuticals but there is a lack of research that compares PNF materials to non-PNF protein materials for water purification. READ MORE

  6. 10. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    University essay from Uppsala universitet/Biokemi

    Author : Sofia Larsson; [2023]
    Keywords : ;

    Abstract : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. READ MORE