Essays about: "protein purification"

Showing result 21 - 25 of 111 essays containing the words protein purification.

  1. 21. Developing a Method to Study the Impact of Microbial DNA on the Brain Related to Alzheimer’s Disease – Can Food Make a Change?

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master)

    Author : Fuh-Tzu Yang; [2022]
    Keywords : DNA extraction; Microbiome DNA enrichment; Host DNA depletion; 16S rRNA sequencing; Alzheimer’s disease; Gut-brain axis; Microbiota; Microbial DNA; Black currant; Applied Nutrition and Food Chemistry; Medicine and Health Sciences;

    Abstract : Understanding the gut-brain-microbiota axis can help us provide a new tool to manage conditions such as Alzheimer’s. However, a major challenge is selectively amplifying the bacterial DNA which is present at a much lower level than the host DNA. READ MORE

  3. 22. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE

  4. 23. Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Håkansson Anja; Dalén Therese; Gröblacher Josefine; Göransson William; Jaksties Jonathan; Ortstad Nathalie; Lenkeit Gesser Pauline; [2022]
    Keywords : Affibody AB; Pichia pastoris; pAOX1; pAOX2; pFLD1; pGAP; cultivation; fermentation; glycosylation;

    Abstract : Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. READ MORE

  5. 24. Double-layer vs single-layer cryogels as a dye affinity chromatography column, comparison study

    University essay from Lunds universitet/Kemiska institutionen

    Author : Thi Hoai Thu Trinh; [2022]
    Keywords : dye-affinity chromatography; single-layer cryogels; double-layer cryogels; protein purification; bovine serum albumin; biochemistry; Chemistry;

    Abstract : Dye affinity chromatography is one of the chromatography techniques which uses dye as a ligand for protein purification. In this study, single-layer poly(acrylamide-allyl glycidyl ether) cryogel was prepared, which contains free epoxy groups at sub-zero temperature. READ MORE

  6. 25. Alkaline Protein Extraction of Oat Bran and Oat Endosperm Flour - the Effect of pH during Extraction and Precipitation

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master)

    Author : Bella Nielsen; Alva Rodhe; [2021]
    Keywords : Oats; Protein; Alkaline extraction; Protein extraction; Plant-based proteins; Oat protein; Oat bran; Oat endosperm; Iso-electric precipitation; Food engineering; Technology and Engineering; Agriculture and Food Sciences;

    Abstract : An increased understanding of the negative impacts of animal-based proteins, on both environment and health, has led to a rise in the demand for plant-based proteins. To meet the demands, conventional crops must be efficiently utilized. READ MORE