Essays about: "solubility tag"

Showing result 1 - 5 of 7 essays containing the words solubility tag.

  1. 1. Recombinant production of spider silk for enzymatic phosphorylation

    University essay from KTH/Proteinvetenskap

    Author : Kristel Jaiu; [2022]
    Keywords : Caddisfly larval silk; FN-4RepCT; phosphorylation; protein kinase A; recombinant spider silk; Nattsländesilke; FN-4RepCT; fosforylering; proteinkinas A; rekombinant spindeltrådsilke;

    Abstract : Ett rekombinant framställt miniatyrspindelsilkeprotein, funktionaliserat med ett Arg-Gly-Asp-motiv som finns i fibronektin, FN-4RepCT, har använts för många olika bioteknologiska tillämpningar, som vävnadsteknik och cellodling, i form av fibrer, filmer och skum. FN-4RepCT kan produceras i Escherichia coli, och det interagerar spontant med sig självt och bildar fibrer under fysiologiska förhållanden. READ MORE

  3. 2. Does SCP-2 promote the expression of foreign proteins in Escherichia coli?

    University essay from Linköpings universitet/Biologi

    Author : Isak Mikkola; [2016]
    Keywords : CAT∆9; Escherichia coli; GFP; inclusion body; pET-15b; SCP-2; solubility tag;

    Abstract : Expression of foreign proteins in host organisms usually results in the development of insoluble, inactive proteins. Further, these proteins have a tendency to form aggregates termed inclusion bodies. However, the formation of inclusion bodies can be avoided by fusing the gene encoding the foreign protein to a highly soluble protein. READ MORE

  4. 3. Can Sterol Carrie Protein-2 function as a solubility tag in E.coli?

    University essay from Linköpings universitet/Biologi

    Author : Amanda Lundén; [2016]
    Keywords : CATΔ9; E.coli; GFP; pET-15b; protein expression; recombinant proteins; SCP-2; Solubility tag;

    Abstract : Expressing foreign proteins in E.coli is a major challenge because they often tend to develop into unsolvable and inactive proteins. They aggregate into so called  inclusion bodies which prevent expression of the protein. READ MORE

  5. 4. Overexpression and Purification of Mouse Divalent Metal Transporter 1 (mDMT1) from Saccharomyces cerevisiae

    University essay from Lunds universitet/Examensarbeten i molekylärbiologi

    Author : Olivia Arbab; [2016]
    Keywords : Biology and Life Sciences;

    Abstract : The membrane integrated divalent metal transporter 1 (DMT1) functions as a transporter of mainly iron across cellular membranes within the human body. Studies indicate that dysregulated transport by DMT1 causes accumulation of iron within the midbrain leading to neuronal cell death – a common symptom among patients suffering from Parkinson’s disease (PD). READ MORE

  6. 5. Recombinant spider silk with antimicrobial properties

    University essay from Institutionen för fysik, kemi och biologi; Tekniska högskolan

    Author : Linnea Nilebäck; [2013]
    Keywords : Spider silk; antimicrobial peptides; bactericidal enzymes; recombinant production; antibacterial assays; Spindeltråd; antimikrobiella peptider; antibakteriella enzymer; rekombinant produktion; antibakteriella analyser;

    Abstract : Immobilizing antimicrobial substances onto biocompatible materials is an important approach for the design of novel, functionalized medical devices. By choosing antimicrobial substances from innate immune systems, the risk for development of resistance in pathogenic microbes is lower than if conventional antibiotics are used. READ MORE