Essays about: "DNA purification"

Showing result 1 - 5 of 28 essays containing the words DNA purification.

  1. 1. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Keywords : Plasmid purification; pDNA; large-scale chromatography;

    Abstract : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. READ MORE

  2. 2. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elnaz Samadian Zad; [2023]
    Keywords : adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Abstract : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. READ MORE

  3. 3. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Anna Králová; [2023]
    Keywords : time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Abstract : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. READ MORE

  4. 4. The Examination of Alternative Methods for Sperm Cell Lysis and Purification of Sexual Assault Samples

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Emilie Boson; [2023]
    Keywords : Differential lysis; Differential extraction; Sexual assault samples; Sperm cells; Forensic; DNA recovery; DNA profiling; DNA purification; Applied microbiology; Technology and Engineering; Biology and Life Sciences;

    Abstract : Forensic sexual assault samples typically contain a mixture of a small quantity of sperm cells and a high quantity of epithelial cells. This relationship complicates the generation of a DNA profile, which prompts the need for differential lysis and extraction of the DNA. READ MORE

  5. 5. Acquired and lost structure-functions of non-enveloped and enveloped viruses

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Diogo Filipe; [2022]
    Keywords : ;

    Abstract : Viruses are biological entities that constantly affect the world around us and in many cases they can harm the health of humans and negatively impact food production, in agriculture andfisheries. The project concept is that, through the structural studies of viruses that infectsimple hosts, it is possible to point out structural features that are unique and conserved inviruses, that infect human, other animals, and crops, in the same phylogenetic lineage. READ MORE