Essays about: "Oligonucleotides"

Showing result 11 - 15 of 24 essays containing the word Oligonucleotides.

  1. 11. The dependence of chromatographic conditions for separation of oligonucleotides in different AEX-HPLC columns

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Julia Nylander; [2020]
    Keywords : ion exchange chromatography; oligonucleotides; anion exchange column; pH; HPLC; Jonbyteskromatografi; oligonukleotider; anjonbytarkolonn; pH; HPLC;

    Abstract : Oligonucleotides (ONs) are widely used in different applications in life science, forensic, in i.e. family tree DNA test for humans and in diagnostic applications in several fields. READ MORE

  3. 12. Optimizing purification of oligonucleotides with reversed phase trityl-on solid phase extraction

    University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

    Author : Ninorta Bartuma; [2019]
    Keywords : Oligonucleotides; purification; trityl-on; SPE; AIE-HPLC;

    Abstract : Oligonucleotides are synthetic strings of DNA or RNA used mostly for biochemical analysis and diagnostics. For them to be useful in these fields, a purity over 90% is most often required. However, when synthesizing these sequences, many “failures” (shorter sequences) are made in the step-wise process. READ MORE

  4. 13. Investigation of pore size effects at separation of oligonucleotides using Ion-pair RP HPLC : Examining of how the particle pore size of the stationary phase affects separations of oligonucleotides in therapeutic range

    University essay from Karlstads universitet/Fakulteten för hälsa, natur- och teknikvetenskap (from 2013)

    Author : Alexander Jonsson; [2019]
    Keywords : Oligonucleotides; Pore size; Stationary phase; Pore diameter; Porosity; DNA oligonucleotides;

    Abstract : Oligonucleotides may become a new class of therapies with the potential of curing many today untreatable diseases. Oligonucleotides becomes increasingly more difficult to separate with an increase in length since the relative difference in retention of these very similar compounds becomes increasingly smaller. READ MORE

  5. 14. Development of separation method for analysis of oligonucleotides using LC-UV/MS

    University essay from Uppsala universitet/Analytisk farmaceutisk kemi

    Author : Björs Ida; [2018]
    Keywords : Oligonucleotides; UPLC; liquid chromatography; time of flight; mass spectrometry; reversed-phase weak anion exchange; AcclaimTM Mixed Mode WAX-1; DNAPacTM RP; ACQUITY UPLC BEH C18;

    Abstract : Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. READ MORE

  6. 15. Effects of herpes simplex virus 1 (HSV-1) infection on nuclear amyloid aggregation

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Maria Arone Blanco; [2018]
    Keywords : Huntington’s disease; Spinocerebellar ataxia; Amyloids; Protein aggregation; Herpes Simplex Virus 1; HSV-1; Virus infection; oligonucleotides; Filter retardation Assay; Dot Blot.; Huntingtons sjukdom; Spinocerebellära ataxier; Amyloider; Protein aggregering; Herpes Simplex Virus 1; HSV-1; Virus infektion; Oligonukleotider; Filter retardation Assay; Dot Blot.;

    Abstract : Huntington’s disease (HD) and Spinocerebellar ataxia (SCA) are incurable neurodegenerative diseases that affect the central nervous system. Amyloids, highly organized protein aggregates, are a hallmark for many neurodegenerative diseases. The presence and accumulation of amyloids are toxic and constitute the major cause of neuron cell death. READ MORE