Essays about: "oligonucleotides"

Showing result 1 - 5 of 16 essays containing the word oligonucleotides.

  1. 1. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

    University essay from Karlstads universitet

    Author : Rebecka Jasinski; [2021]
    Keywords : oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

    Abstract : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. READ MORE

  2. 2. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    University essay from Karlstads universitet

    Author : Lewis Frazer; [2021]
    Keywords : Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Abstract : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. READ MORE

  3. 3. The dependence of chromatographic conditions for separation of oligonucleotides in different AEX-HPLC columns

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Julia Nylander; [2020]
    Keywords : ion exchange chromatography; oligonucleotides; anion exchange column; pH; HPLC; Jonbyteskromatografi; oligonukleotider; anjonbytarkolonn; pH; HPLC;

    Abstract : Oligonucleotides (ONs) are widely used in different applications in life science, forensic, in i.e. family tree DNA test for humans and in diagnostic applications in several fields. READ MORE

  4. 4. Optimizing purification of oligonucleotides with reversed phase trityl-on solid phase extraction

    University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

    Author : Ninorta Bartuma; [2019]
    Keywords : Oligonucleotides; purification; trityl-on; SPE; AIE-HPLC;

    Abstract : Oligonucleotides are synthetic strings of DNA or RNA used mostly for biochemical analysis and diagnostics. For them to be useful in these fields, a purity over 90% is most often required. However, when synthesizing these sequences, many “failures” (shorter sequences) are made in the step-wise process. READ MORE

  5. 5. Investigation of pore size effects at separation of oligonucleotides using Ion-pair RP HPLC : Examining of how the particle pore size of the stationary phase affects separations of oligonucleotides in therapeutic range

    University essay from Karlstads universitet/Fakulteten för hälsa, natur- och teknikvetenskap (from 2013)

    Author : Alexander Jonsson; [2019]
    Keywords : Oligonucleotides; Pore size; Stationary phase; Pore diameter; Porosity; DNA oligonucleotides;

    Abstract : Oligonucleotides may become a new class of therapies with the potential of curing many today untreatable diseases. Oligonucleotides becomes increasingly more difficult to separate with an increase in length since the relative difference in retention of these very similar compounds becomes increasingly smaller. READ MORE