Essays about: "affinity ligands"

Showing result 1 - 5 of 16 essays containing the words affinity ligands.

  1. 1. Optimization of two high-performance size-exclusion chromatography methods with diode-array detection for protein quantification and purity assessment

    University essay from Lunds universitet/Centrum för analys och syntes

    Author : Camilla Lane; [2023]
    Keywords : Method qualification; Protein concentration; Protein Purity; High Performance Size exclusion chromatography; Quality control; Analytical chemistry; Biology and Life Sciences;

    Abstract : Background: There is a need to develop methods of determining concentration and purity for two new affinity ligands, protein T and protein H, in order to ensure the quality of an industrial production of said proteins. Aims: To develop a High Performance Size Exclusion Chromatography, HPSEC, method to determine concentration and purity of protein T and to improve an existing HPSEC method for concentration and purity determination of protein H that show selectivity, display linearity between peak area and injection amount and give repeatable results. READ MORE

  3. 2. Bioinformatics analysis on the drug design supporting systems

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Emilia Guszpit; [2023]
    Keywords : ;

    Abstract : This research project investigates the interactions of staurosporine, a potent kinase inhibitor, with 11 ligands, highlighting its role in drug design and bioinformatics. Focusing on the selectivity and promiscuity of staurosporine in binding to protein kinases, the study employs the MANORAA database for data extraction. READ MORE

  4. 3. Drug Design of β-Lactamase Inhibitors of the DBO-scaffold against OXA-48 : A Molecular Dynamics Study of Ligand Stability in the Michaelis Complex

    University essay from Uppsala universitet/Läkemedelsdesign och läkemedelsutveckling

    Author : Linda Liljeholm; [2022]
    Keywords : OXA-48; β-lactamase; DBO inhibitors; diazabicyclooctane; avibactam; relebactam; β-lactamase inhibitor; BLI; molecular dynamics; LIE; Linear Interaction Energy; antibiotic resistance; drug design;

    Abstract : The emergence of β-lactamase-mediated antibiotic resistance is one of the biggest threats in modern times. Combined with the discovery void of new forms of antibiotics, this sets the course toward a future where the efficacy of present-day health care will be jeopardized. READ MORE

  5. 4. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE

  6. 5. Characterization of Alcohol Modulation of a Pentameric Ligand-gated Ion Channel with Electrophysiology and Molecular Dynamics Simulations

    University essay from KTH/Tillämpad fysik

    Author : Sabina Gutheim; [2021]
    Keywords : Biophysics; Electrophysiology; MD simulations; Ligand-gated ion channels; GLIC; Ethanol modulation; Biofysik; Elektrofysiologi; MDsimuleringar; Pentameriska ligandstyrda jonkanaler; GLIC; Etanolmodulering;

    Abstract : Pentameric ligand-gated ion channels (pLGICs) are membrane receptors that play a crucial role in every living organism. The pLGIC protein structure forms a pore through the membrane of a cell that can let specific ions pass through, upon activation by endogenous agonists. READ MORE