Essays about: "multiplex real-time PCR"

Showing result 1 - 5 of 7 essays containing the words multiplex real-time PCR.

  1. 1. Development of real-time RT-PCR for the detection of human sapovirus in foods

    University essay from SLU/Dept. of Microbiology

    Author : Eva Edlund Tjernberg; [2014]
    Keywords : real-time RT-PCR; sapovirus; TaqMan MGB probe; food-poisoning;

    Abstract : Food-poisoning is a major health problem and an estimated half a million Swedes are food-poisoned annually, with acute gastroenteritis as a consequence. One of the major causes of contaminated foods is related to food- and waterborne viruses. READ MORE

  3. 2. Development of a multiplex real time PCR assay to target bacteria causing meningitis

    University essay from Klinisk mikrobiologi och infektionsmedicin

    Author : Johan Lagmo; [2014]
    Keywords : ;

    Abstract : .... READ MORE

  4. 3. Optimization of the multiplexed Proximity Ligation Assay for detection of blood-based biomarkers

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Martin Lundberg; [2014]
    Keywords : PLA; colorectal cancer; biomarker; multiplex qPCR; Olink;

    Abstract : The Proximity Ligation Assay (PLA) is a relatively new method which utilizes the strength of both immunoassays and DNA detection. PLA has the capacity of high multiplexing due to the high specificity achieved with both dual protein-binding and dual primer binding during detection with Real-Time PCR. READ MORE

  5. 4. Optimization and validation of a triplex real-time PCR assay for thermotolerant Campylobacter species associated with foodborne disease

    University essay from SLU/Dept. of Microbiology

    Author : Maria Karlsson; [2013]
    Keywords : thermotolerant Campylobacter; multiplex real-time PCR; 16S rRNA; hipO; cadF;

    Abstract : The genus Campylobacter is globally recognised as the leading bacterial cause of human foodborne gastroenteritis. Every year around 8000 Swedes are infected by Campylobacter. Most people are infected by thermotolerant Campylobacter species, commonly C. jejuni and C. READ MORE

  6. 5. Development of a PCR method to detect HLA-B27 in ankylosing spondylitis

    University essay from Institutionen för medicinsk biokemi och mikrobiologi

    Author : Ylva Nätterkvist; [2012]
    Keywords : HLA; Multiplex PCR; DNA extraction; hot-start Taq; Spondyloarthropathies;

    Abstract : The aim of the project was to develop a PCR method to detect HLA-B27 at the Immunology Department of St. James hospital in Dublin. The HLA-B27 gene is common among patients with ankylosing spondylitis (AS). READ MORE