Essays about: "single-particle cryo-EM"

Found 3 essays containing the words single-particle cryo-EM.

1. 1. Engineered imaging scaffolds for cryo-EM of small proteins of interest

   University essay from KTH/Proteinvetenskap

   Author : Oscar Friberg; [2022]
   Keywords : Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

   Abstract : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. READ MORE

3. 2. Design, production and evaluation of cross linked target proteins to an affibody-based carrier framework aimed for affinity protein: antigen structure determination using single particle Cryo-EM

   University essay from KTH/Proteinvetenskap

   Author : Richard Brunsell; [2021]
   Keywords : Cryo-EM; Structure determination; Protein A; Affibody; Aldolase; Scaffold; Rigid helix fusion; Photoconjugation; UV crosslinking; BPA;

   Abstract : Small proteins are difficult to study at high resolution with single-particle cryo-electron microscopy (cryo-EM). In general, sample properties such as large size (> 80 kDa), symmetry and rigidity are key to utilize this technology. READ MORE

4. 3. Geometric algebra, conformal geometry and the common curves problem

   University essay from KTH/Skolan för teknikvetenskap (SCI)

   Author : Elias Riedel Gårding; [2017]
   Keywords : ;

   Abstract : This bachelor’s thesis gives a thorough introduction to geometric algebra (GA), an overview of conformal geometric algebra (CGA) and an application to the processing of single particle data from cryo-electron microscopy (cryo-EM). The geometric algebra over the vector space Rp;q, i.e. READ MORE