Weight losses of Green tea and Rooibos tea in an aquatic environment : The importance of leaching when estimating decomposition rates

Abstract: Leaching is one of the major processes occurring when organic litter is decomposed and is often completed within a few days when litter enters aquatic environments. It is important that leaching is addressed when studying microbial and invertebrate decomposition rates in order to avoid overestimations. The traditional litter bag method that has been used to measure decomposition rates in both terrestrial and aquatic environments has in recent years been challenged by the new and widely adopted tea bag index (TBI). Both methods, however, fail to bring a standardized methodology for separating and recognizing weight losses of litter due to leaching and biotic decomposition. Through a field experiment in two streams with different water discharge, this study has focused on exploring the leaching phase and post-leaching phase of the tea products used in TBI. The results unveiled that 20% of rooibos tea’s and 44% of green tea’s initial weight was lost to leaching within three days (72 hours) of the experiment. After the 72nd hour, both teas remained in a stabilized phase until the end of the experiment (120 hours). Water discharge had no significant effect on neither of the tea-weights during or after the leaching phase. This study recommends that weight loss through the leaching phase are taken into account in future studies and advocate the development of an updated TBI protocol where leaching losses are recognized. If not, overestimations of active decomposition rates will be made and may result in compromised conclusions.