Essays about: "3´ UTR"

Showing result 1 - 5 of 7 essays containing the words 3´ UTR.

  1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Alexander Westlund; [2019]
    Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

  3. 2. Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells

    University essay from Linköpings universitet/Teknisk biologi

    Author : Ellen Einarsson; [2018]
    Keywords : Regulation; Untranslated regions; 5´ UTR; 3´ UTR; mRNA; Expression; Flow cytometry;

    Abstract : The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein. READ MORE

  4. 3. Structural elucidation of mRNA(Sirt1)-microRNA 34a complex

    University essay from KTH/Skolan för teknikvetenskaplig kommunikation och lärande (ECE)

    Author : Mona Farshchian; [2015]
    Keywords : mRNA Sirt1 ; miR-34a; in vitro transcription; gel electrophoresis; CD spectroscopy; NMR; cancer regulation via p53; HPLC; mRNA Sirt1 ; miR-34a; in vitro transkription; gel elektroforesis; CD spektroskopi; NMR; cancer regulering via p53; HPLC;

    Abstract : The aim of this thesis is to understand RNA-RNA interactions steering cellular functions, as in the case of this thesis the structure of mRNA(Sirt1) in complex with microRNA-34a (miR-34a). MiR-34a regulates the cancer protein p53 via Sirt1 modulation. READ MORE

  5. 4. Functional Analysis of PTPN3 associated with Immune-Mediated Rheumatic Disease in Nova Scotia Duck Tolling Retrievers

    University essay from SLU/Dept. of Animal Breeding and Genetics

    Author : Pedro Sousa; [2013]
    Keywords : lupus; gene expression; genetic association; cloning; luciferase assay;

    Abstract : This study investigates whether risk haplotypes of highly associated regions in PTPN3 gene in dogs suffering from immune mediated rheumatic disease, might affect the regulatory potential and expression levels of this gene influencing the phenotype. The overall aim of this thesis is to identify causative mutations that may alter PTPN3 gene expression in NSDTRs with risk for IMRD. READ MORE

  6. 5. Analysis of conserved microRNA targets in the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster.

    University essay from Institutionen för naturvetenskap, miljö och teknik

    Author : Ninwa Youssef; [2013]
    Keywords : ;

    Abstract : MicroRNA (miRNA) is small regulatory non-coding single stranded RNA molecule that can repress protein expression either at transcriptional or translational level. Since their discoveries in nematodes in the early 1990´s extensive research have shown that this mechanism is conserved across species. READ MORE