Essays about: "Affibody"

Showing result 6 - 10 of 23 essays containing the word Affibody.

  1. 6. Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Håkansson Anja; Dalén Therese; Gröblacher Josefine; Göransson William; Jaksties Jonathan; Ortstad Nathalie; Lenkeit Gesser Pauline; [2022]
    Keywords : Affibody AB; Pichia pastoris; pAOX1; pAOX2; pFLD1; pGAP; cultivation; fermentation; glycosylation;

    Abstract : Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. READ MORE

  3. 7. Affibody phage display selections for lipid nanoparticle and affibody-mediated transient CAR T-cell therapy

    University essay from KTH/Proteinvetenskap

    Author : Tasnim Yasin Idris; [2022]
    Keywords : affibody; phage display; in vitro selection; CD5; CD8; CD19; CAR T-cell therapy.;

    Abstract : CAR T-cellbehandling är en immunterapi som har visat lovande resultat vid behandling av cancer. Trots det riktade immunsvaret som kan uppnås, betonar komplexiteten i tillverkningsprocessen och behandlingsproceduren det utrymme somm finns för förbättringar. READ MORE

  4. 8. Development of an AVI-tagged phagemid vector

    University essay from KTH/Proteinvetenskap

    Author : Barathram Swaminathan; [2022]
    Keywords : phage display; affibody; directed evolution; protein engineering; protein interactions; fagdisplay; affibody; riktad evolution; protein engineering; proteininteraktioner;

    Abstract : Fagmider är ett kraftfullt verktyg som använts för att uttrycka heterologa proteiner på fagvirus i metoder som exempelvis riktad evolution via fagdisplay av proteinbinliotek. Monovalent uttryck genom fagemider lider av ett överflöd av kala fager som är svåra att rensa bort. READ MORE

  5. 9. Development of an Affibody-based Prodrug Against HER2 for Cancer Therapy

    University essay from KTH/Proteinvetenskap

    Author : Cornelia Westerberg; [2021]
    Keywords : Affibody; therapeutic protein; prodrug; affibody-based prodrug; HER2-targeted therapy;

    Abstract : Affinity proteins constitute an important category of cancer therapeutics. Owing to properties such as high target affinity and selectivity, therapeutic proteins offer more targeted therapy than small molecule drugs. The target molecules are typically proteins that are overexpressed on the surface of tumour cells, such as membrane-bound receptors. READ MORE

  6. 10. Design, production and evaluation of cross linked target proteins to an affibody-based carrier framework aimed for affinity protein: antigen structure determination using single particle Cryo-EM

    University essay from KTH/Proteinvetenskap

    Author : Richard Brunsell; [2021]
    Keywords : Cryo-EM; Structure determination; Protein A; Affibody; Aldolase; Scaffold; Rigid helix fusion; Photoconjugation; UV crosslinking; BPA;

    Abstract : Small proteins are difficult to study at high resolution with single-particle cryo-electron microscopy (cryo-EM). In general, sample properties such as large size (> 80 kDa), symmetry and rigidity are key to utilize this technology. READ MORE