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Showing result 1 - 5 of 6 essays matching the above criteria.
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1. Understanding sequence errors in analysis of short tandem repeats through optimisation of a library preparation protocol utilising unique molecular indices
University essay from Uppsala universitet/Institutionen för biologisk grundutbildningAbstract : In the last few years massively parallel sequencing (MPS) has become important for forensic DNA analysis by providing new and broader possibilities, such as increased discrimination power for short tandem repeat (STR) analyses. An imperative part of MPS analysis is library preparation where there is a fundamental reliability on in vitro DNA polymerisation, which is prone to errors. READ MORE
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2. Optimisation of PCR Protocol for Microsatellites in Vaccinium myrtillus : A first step in evaluating genetic diversity for future conservation
University essay from Linköpings universitet/BiologiAbstract : A growing world population means an increase in crop demand. At the same time climate change threatens food security as crops may become maladapted to a new environment. We need to adapt crops to increase crop yield and become resistant to a changed environment to meet this. READ MORE
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3. Optimisation and Validation of PCR Method for HLA Gene Expression to Enable PCR System Transfer and Master Mix Change
University essay from Linköpings universitet/Institutionen för fysik, kemi och biologiAbstract : Health Tech company Dynamic Code AB provides a PCR test for determination of HLA DQ-genes connected to development of celiac disease. The PCR method is probe based and in real time and is at this time carried through on the, somewhat outdated, PCR instrument from Thermo Fisher/Applied Biosystems called 7300 Real-Time PCR System. READ MORE
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4. PCR Optimisation and Sequencing of L1CAM for the Verification of a Mutation in a Family with L1 Syndrome
University essay from Institutionen för klinisk och experimentell medicinAbstract : L1 syndrome is an X-linked recessive disorder, characterised by congenital hydrocephalus, adducted thumbs, spastic paraplegia, agenesis of the corpus callosum and mental retardation. The disease is caused by mutations in the L1CAM gene, encoding a protein predominantly expressed in the nervous system. READ MORE
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5. Trametes versicolor laccase: random mutagenesis and heterologous expression in Pichia pastoris
University essay from Institutionen för kemiAbstract : Laccase is a blue multi-copper oxidase. It has a broad biotechnical potential which increases the interest to study the enzyme further. A laccase-encoding gene from the white-rot fungus Trametes versicolor (lcc2) was mutated using two different methods for random mutagenesis: error-prone PCR and a method based on an E. READ MORE
