Essays about: "genetic manipulation"

Showing result 1 - 5 of 10 essays containing the words genetic manipulation.

  1. 1. Genetic manipulation to improve efficacy of dendric cell adoptive immunotherapy against cancer in dogs

    University essay from Karlstads universitet

    Author : Felicia Berglund; [2023]
    Keywords : Adoptive immunotherapi;

    Abstract : To improve the efficacy of the dendritic cell vaccine Alv B DC from Alv B, the PD-L1 expression in cancer cells was attempted to be reduced through a transfection with a custom designed siRNA. Before transfecting the dendritic cells, the siRNA functionality had to be tested through flow cytometry, that resulted in negative results and therefore led to a RT-qPCR protocol that indicated that the siRNA was functional. READ MORE

  3. 2. Evaluation of strategies for construction of protease libraries in yeast.

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Author : Sidharth Jaya Sankar; [2022]
    Keywords : Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Abstract : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. READ MORE

  4. 3. Assessment of promoter regulation in Pseudomonas putida using GFP and flow cytometry

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Luka Cululejevic; [2022]
    Keywords : applied microbiology; Pseudomonas putida; promoter characterization; aromatic compounds; vanillin; guaiacol; Biology and Life Sciences;

    Abstract : Lignin is found in all biomass and is an abundant natural source of aromatic compounds. It has the potential to serve as a renewable raw material for a variety of chemicals currently derived from fossil fuel sources such as muconic acid, an important platform chemical. Kraft lignin is a waste stream from the pulp and paper industry. READ MORE

  5. 4. Designing an optmized met X/Y enzymes for an efficient methionine production in E.coli

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Luísa Rodrigues; [2022]
    Keywords : ;

    Abstract : Methionine is a proteinogenic amino acid critical amino for the pharmaceutical, biotechnological and agricultural industries. The overall methionine biosynthetic pathway is as follows: 1. Acetylation of homoserine and its subsequent activation 2. Formation of homoserine through the replacement hydroxyl group by a thiol group, 3. READ MORE

  6. 5. Embryotransfer : en framtid för svensk fårnäring?

    University essay from SLU/Dept. of Animal Environment and Health

    Author : Elinor Asp Tauni; [2017]
    Keywords : embryotransfer; MOET; får; Sverige; import;

    Abstract : Embryotransfer innebär att ett embryo överförs från en donator till en mottagare. Denna teknik gör att man kan öka antalet avkommor från ett moderdjur och vid import kan transporten av levande djur undvikas och ett högre smittskydd nås. READ MORE