Essays about: "molecular microbiology"

Showing result 1 - 5 of 13 essays containing the words molecular microbiology.

1. 1. Evaluation of strategies for construction of protease libraries in yeast.

   University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

   Author : Sidharth Jaya Sankar; [2022]
   Keywords : Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

   Abstract : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. READ MORE

3. 2. Expression of FLAG-tagged argonautes in Dictyostelium discoideum

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för cell- och molekylärbiologi

   Author : Zozek Abdul Rahman; [2022]
   Keywords : Dictyostelium; discoideum; Argonaute; Protein; FLAG; FLAG-tag; Molecular Biology; Microbiology; SorMC; agnA; agnC; agnE; electrophoresis; pDM304;

   Abstract : Argonautes are conserved RNA-binding proteins that can regulate gene expression post transcriptionally through a process known as RNA interference (RNAi). This is done through the use of small RNAs, e.g. sRNAs that act as a guide for the argonautes, allowing for sequence-specific binding to the target site. READ MORE

4. 3. Development of a Near Infrared Spectroscopy Model for Prediction of Fibre Compounds in Alfalfa

   University essay from Lunds universitet/Teknisk mikrobiologi

   Author : Christina Andersen; [2020]
   Keywords : alfalfa; lucerne; medicago sativa; green protein powder; NIR; near infrared spectroscopy; model development; matlab; PLS; partial least squares; PCA; principal component analysis; SNV; standard normal variate; fibre prediction; TDF; total dietary fibre; applied microbiology; teknisk mikrobiologi; Technology and Engineering;

   Abstract : Background: This project investigates if it is possible to develop a calibration model from near infrared (NIR) spectroscopic measurements, for determination of the amount and type of fibre fractions within protein powder produced from the legume alfalfa, without performing wet experiments. Alfalfa is also known as Medicago sativa and lucerne, but is in this project further referred to as alfalfa. READ MORE

5. 4. Library construction optimization and analysis of Short Tandem Repeats by a simple, PCR-based DNA barcoding method

   University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

   Author : Markus André Soma; [2020]
   Keywords : Forensics; DNA; Short Tandem Repeats; Unique Molecular Identifiers; Polymerase Chain Reaction; Capillary Electrophoresis; Massively Parallel Sequencing; Applied Microbiology; Teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

   Abstract : DNA profiling is evolving in the forensics community towards introducing massively parallel sequencing (MPS) as a complement to capillary electrophoresis (CE). Obstacles remain however before this technology can become routine in forensic casework, such as the development of more efficient bioinformatics solutions and recommendations concerning data analyses. READ MORE

6. 5. Assessment of the Fluorescent Activities of Different Fluorescent Proteins for Potential Use as Biosensors in Saccharomyces cerevisiae

   University essay from Lunds universitet/Teknisk mikrobiologi

   Author : Johan Davidsson; [2020]
   Keywords : Fluorescent proteins; Saccharomyces cerevisiae; Flow cytometry; CRISPR Cas9; Biosensors; Applied microbiology; Tillämpad mikrobiologi; Technology and Engineering;

   Abstract : Development of genetically modified microorganisms such as Saccharomyces cerevisiae for use as microbial cell factories to produce fine and bulk chemicals has lately proceeded very rapidly, much thanks to new gene editing methods based on a CRISPR/Cas9 system adapted from bacteria which increases the rate of genetic engineering. However, development of methods which enable fast and simple screening of the best performing strains with specific sought-after characteristics is not advancing at the same pace. READ MORE