Essays about: "Fluorescerande protein"

Showing result 1 - 5 of 14 essays containing the words Fluorescerande protein.

  1. 1. Indocyanine-green fluorescence imaging to detect anastomotic insufficiency after esophagectomy

    University essay from Lunds universitet/Fysiska institutionen; Lunds universitet/Förbränningsfysik

    Author : Love Kildetoft; [2024]
    Keywords : Fluorescence; Imaging; Indocyanine-green; ICG; Esophageal cancer; Physics; Photonics; Image Analysis; Blood Perfusion; Anastomotic leakage; Anastomotic insufficiency; Physics and Astronomy;

    Abstract : This research project is concerned with the development of simulation models and image analysis algorithms, as well as instrumentation for characterizing anastomotic leakages after a patient has undergone surgery for esophageal cancer. Anastomotic leakage is a high-incidence complication after esophagectomy, a surgical procedure for esophageal cancer. READ MORE

  3. 2. Transient state UV spectroscopy of Tyrosine and Tyrosine-containing protein

    University essay from KTH/Tillämpad fysik

    Author : Hongjian Chen; [2023]
    Keywords : Tyrosine; Calmodulin; Autofluorescence; Transient-state monitoring; Electronic state transitions; Tyrosin; Calmodulin; Autofluorescens; Transient-tillståndsövervakning; Elektroniska tillståndsövergångar;

    Abstract : The aromatic amino acids tryptophan, tyrosine, and phenylalanine have been extensively used for different label-free protein studies. These investigations extract information on protein conformations and interactions from the emitted fluorescence's intensity, wavelength, and/or polarization. READ MORE

  4. 3. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE

  5. 4. Application of Biomimetic Membrane Models in Expansion Microscopy

    University essay from KTH/Proteinvetenskap

    Author : Praghadhesh Thiagarajan; [2023]
    Keywords : Fluorescence microscopy; expansion microscopy; GPMV; inter-leaflet asymmetry; Fluorescensmikroskopi; expansionsmikroskopi; GPMV; asymmetri mellan lagren;

    Abstract : Plasmamembranet är en komplex struktur som består av biomolekyler som lipider, proteiner och glykaner. Membranets rena komplexitet begränsar vår förmåga att förstå den spatiotemporala dynamiken hos sådana komponenter och deras kollektiva biofysiska membranparametrar. READ MORE

  6. 5. The use of Gibson Assembly for DNA cloning

    University essay from KTH/Tillämpad fysik

    Author : Samuel Johansson; [2022]
    Keywords : Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

    Abstract : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. READ MORE