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Found 3 essays matching the above criteria.

  1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Alexander Westlund; [2019]
    Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

  3. 2. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    University essay from Linköpings universitet/Teknisk biologi

    Author : Malin Strannermyr; [2018]
    Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE

  4. 3. Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells

    University essay from Linköpings universitet/Teknisk biologi

    Author : Ellen Einarsson; [2018]
    Keywords : Regulation; Untranslated regions; 5´ UTR; 3´ UTR; mRNA; Expression; Flow cytometry;

    Abstract : The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein. READ MORE