Essays about: "gene knockout"

Showing result 21 - 23 of 23 essays containing the words gene knockout.

1. 21. Troubleshooting the GFP-tagging gene knockout (GGKO) method for the Leptosphaeria maculans effectors AvrLm6 and AvrLm4-7

   University essay from SLU/Dept. of Plant Biology and Forest Genetics (until 131231)

   Author : Carl-Fredrik Johnzon; [2011]
   Keywords : Leptosphaeria maculans; AvrLm6; AvrLm4-7; GFP; GGKO;

   Abstract : An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined for potential errors. READ MORE

3. 22. Effect of protein phosphatase gene sppA deletion on hyphal growth in Streptomyces coelicolor when combined with different protein kinase gene afsK mutations

   University essay from Lunds universitet/Kemiska institutionen

   Author : Juri Kazakevych; [2011]
   Keywords : Biokemi; Chemistry;

   Abstract : Streptomyces species are known for their bacteria-untypical growth and sporulation. Growth by tip extension building up a mycelium consisting of long branching hyphae is a highly polar and regulated process. An essential protein invalved in hyphal growth and branching regulation is DiviVA. In S. READ MORE

4. 23. mRNA and natural endogenous antisense RNA in Dictyostelium discoideum

   University essay from SLU/Dept. of Molecular Biosciences

   Author : Louise Andersson; [2006]
   Keywords : RNAi; mRNA; antisense RNA; Dictyostelium discoideum;

   Abstract : The aim of this Master's Thesis was to investigate the presence and regulation of RNAi in the model organism Dictyostelium discoideum. The background for the project is a cDNA library representing 18-25nt RNAs (9000 clones) from Dictyostelium discoideum that recently was constructed. READ MORE