Advanced search

Showing result 16 - 18 of 18 essays matching the above criteria.

  1. 16. Generating large-scale conditional knock-in mouse models using CRISPR/Cas9 in embryonic stem

    University essay from SLU/Dept. of Animal Breeding and Genetics

    Author : Gus Rowan Mcfarlane; [2016]
    Keywords : CRISPR Cas9; conditional knock-in mice; transgenic mice; Fbxw7; colorectal cancer;

    Abstract : The discovery and repurposing of CRISPR/Cas9 has revolutionized the field of genome editing and allowed for the development of mouse models with unprecedented simplicity and speed. The application of CRISPR/Cas9 in mouse embryonic stem cells represents a highly efficient and wellestablished method for generating large-scale conditional knock-in mouse models. READ MORE

  3. 17. CRISPR Cas9 system for plant genome editing within the European Union

    University essay from SLU/Department of Plant Biology (from 140101)

    Author : Ida Eriksson; [2015]
    Keywords : Arabidopsis thaliana; genome editing; European Union; CRISPR Cas9;

    Abstract : CRISPR (clustered regularly interspaced short palindromic repeat) Cas9 systems is a novel technique for genome editing. In this thesis a start-up attempt using CRISPR Cas9 to induce site-specific mutations in Arabidopsis thaliana has been made. READ MORE

  4. 18. Agroinfiltration och transient uttryck av CRISPR/Cas9 : en förberedande studie på Nicotiana benthamiana och potatis (Solanum tuberosum L.)

    University essay from SLU/Dept. of Biosystems and Technology (from 130101)

    Author : Niklas Olsson; [2014]
    Keywords : CRISPR; Cas9; Agrobacterium; agroinfiltration; Nicotiana; potatis; potatis; in-vivo mutation;

    Abstract : Den senast utvecklade tekniken för riktade förändringar av genom in-vivo är baserad på CRISPR/Cas-systemet för vilket förutsättningarna i Nicotiana benthamiana och potatis, Solanum tuberosum, här undersöktes. För att föra in genkonstruktioner i växterna användes Agrobacterium tumefaciens som via vakuum kommer i kontakt med de inre delarna av bladen varefter de gener som finns på en binär vektor uttrycks transient. READ MORE