Essays about: "host cell protein"

Showing result 11 - 15 of 35 essays containing the words host cell protein.

  1. 11. Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Håkansson Anja; Dalén Therese; Gröblacher Josefine; Göransson William; Jaksties Jonathan; Ortstad Nathalie; Lenkeit Gesser Pauline; [2022]
    Keywords : Affibody AB; Pichia pastoris; pAOX1; pAOX2; pFLD1; pGAP; cultivation; fermentation; glycosylation;

    Abstract : Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. READ MORE

  3. 12. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans

    University essay from Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Author : Aakash Reddy Murugadasan; [2022]
    Keywords : Gluconobacter oxydans; Escherichia coli; 5-Keto-D-gluconic acid; Alcohol dehydrogenase; Biotechnology; Biology and Life Sciences;

    Abstract : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. READ MORE

  4. 13. Building Tolerance to Gut Bacteria: Quantification of Goblet Cell – Immune Interactions in the Proximal and Distal Colon

    University essay from Lunds universitet/Examensarbeten i molekylärbiologi

    Author : Nellie Asseh Hall; [2021]
    Keywords : Biology and Life Sciences;

    Abstract : Goblet cell - immune cell interactions and their role in inflammatory bowel disease Goblet cells, specialized mucus secreting cells lining the gut, are key players in mediating immune responses. While the mucus layer functions as a barrier against the intrusion of foreign bacteria into the host, substances from e. READ MORE

  5. 14. Structural studies of Arabidopsis Chloroplast Glutamyl Endopeptidase (CGEP)

    University essay from Uppsala universitet/Molekylär biofysik; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Shruti Ravi; [2021]
    Keywords : structural studies; peptidases;

    Abstract : Peptidases play an essential role in cell machinery by regulating protein turnover and processing synthesized proteins for cellular transport. The chloroplast is a complex organelle present in plants that host several peptidases. READ MORE

  6. 15. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Farhana Manzoor; [2020]
    Keywords : Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

    Abstract : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. READ MORE