Essays about: "host cell protein"

Showing result 16 - 20 of 35 essays containing the words host cell protein.

  1. 16. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Eva Sjöblom; [2020]
    Keywords : BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Abstract : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. READ MORE

  3. 17. Intratumoral heterogeneity in early breast cancer

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : MARIA STOGIANNITSI; [2020]
    Keywords : breast cancer; RNAscope technique; heterogeneity of BC; RNA in situ hybridization;

    Abstract : The use of adjuvant The use of adjuvant polychemotherapy (ACT) confers unequivocal benefits in terms of relapse free (RFS) and overall survival (OS) after resection of early breast cancer (BC). Although the magnitude of benefit is the same regardless of clinicopathological factors such as ER expression or nodal stage, it is clear that a substantial proportion of patients will eventually relapse and succumb to the disease. READ MORE

  4. 18. Investigation of immunomodulatory properties of anti-secretory factor in experimental brain tumor models

    University essay from Lunds universitet/Läkarutbildningen

    Author : Hanin Fabiana El-Sabee; Amanda Peetre; [2020]
    Keywords : Antisecretory factor; Immunomodulation; Glioblastoma; Medicine and Health Sciences;

    Abstract : Introduction: Glioblastoma is the most aggressive form of primary brain tumor in adults. Current treatment including chemotherapy poses a limitation due to consequences of systemic toxicity. Further studies for alternative, multimodal treatments are necessary. Antisecretory factor is an endogenous protein. READ MORE

  5. 19. Exploration of Zinc finger CCCH domain-containing protein 11A’s role in mammalian cell NFkB Pathway

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Jianxiang Wang; [2019]
    Keywords : ZC3H11A; NFkB; HeLa cells; Viral-host interaction;

    Abstract : ZC3H11A (ZC3) protein has been reported to be part of the TREX (TRanscription-EXport) nuclear export system for mammalian cells. According to our previous publication, ZC3 not only plays an unelucidated role in the TREX complex, but also supports the growth of several human nucleus replicating virus, such as influenza virus, adenovirus (HAdV), herpes simplex virus and HIV. READ MORE

  6. 20. Cloning and Expression of TBEV NS4A in A549 cells

    University essay from Örebro universitet/Institutionen för medicinska vetenskaper

    Author : Lovisa Gerdhem; [2019]
    Keywords : ;

    Abstract : Background: Tick borne Encephalitis Virus (TBEV), the cause of the fatal Central Nervous System (CNS) infection Tick Borne Encephalitis (TBE), belongs to the family of Flaviviridae and the genusFlavivirus. In order to replicate themselves, they cause the formation of a replication complex, which is nestled inside vesicles of the Endoplasmic Reticulum (ER) membrane to protect the RNA from host defense mechanisms. READ MORE