Essays about: "protein scaffold"

Showing result 1 - 5 of 21 essays containing the words protein scaffold.

  1. 1. The role of AmotL2 in the regulation of mesenchymal transitioning of endothelial cells

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Anita-Ann Monteiro; [2023]
    Keywords : Endothelial-mesenchymal transition EndMT ; atherosclerosis; transforming growth factor β TGF-β ; Angiomotin-Like 2 AmotL2 ; Scrambled Scr ; knockdown kd ; Immunofluorescence IF ;

    Abstract : Background During development, endothelial cells acquire mesenchymal-like properties to migrate and facilitate normal vascular formation. This process of transformation is known as endothelial to mesenchymal transition (EndMT) and has also been implicated in diseases like vascular pathologies contributing to endothelial inflammation, atherosclerosis and tumour angiogenesis. READ MORE

  3. 2. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    University essay from Uppsala universitet/Biokemi

    Author : Sofia Larsson; [2023]
    Keywords : ;

    Abstract : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. READ MORE

  4. 3. Engineered imaging scaffolds for cryo-EM of small proteins of interest

    University essay from KTH/Proteinvetenskap

    Author : Oscar Friberg; [2022]
    Keywords : Protein structure determination; single-particle cryo-EM; imaging scaffold; size limitation problem; affibody; YgjG.;

    Abstract : Strukturbestämning av proteiner är viktigt för att kunna förstå deras funktion och en snabbt utvecklande metod inom fältet är kryoelektronmikroskopi. Storleksbegränsningar förhindrar en bredare applikation av metoden eftersom små proteiner har för låg signal i förhållande till bakgrund för att kunna visualiseras som enstaka partiklar i elektronmiksoskopibilder. READ MORE

  5. 4. Drug Design of β-Lactamase Inhibitors of the DBO-scaffold against OXA-48 : A Molecular Dynamics Study of Ligand Stability in the Michaelis Complex

    University essay from Uppsala universitet/Läkemedelsdesign och läkemedelsutveckling

    Author : Linda Liljeholm; [2022]
    Keywords : OXA-48; β-lactamase; DBO inhibitors; diazabicyclooctane; avibactam; relebactam; β-lactamase inhibitor; BLI; molecular dynamics; LIE; Linear Interaction Energy; antibiotic resistance; drug design;

    Abstract : The emergence of β-lactamase-mediated antibiotic resistance is one of the biggest threats in modern times. Combined with the discovery void of new forms of antibiotics, this sets the course toward a future where the efficacy of present-day health care will be jeopardized. READ MORE

  6. 5. Pipeline for Next Generation Sequencing data of phage displayed libraries to support affinity ligand discovery

    University essay from Uppsala universitet/Institutionen för immunologi, genetik och patologi

    Author : Ella Schleimann-Jensen; [2022]
    Keywords : NGS; next generation sequencing; phage display; affinity ligands; data analysis; bioinformatics;

    Abstract : Affinity ligands are important molecules used in affinity chromatography for purification of significant substances from complex mixtures. To find affinity ligands specific to important target molecules could be a challenging process. Cytiva uses the powerful phage display technique to find new promising affinity ligands. READ MORE