Essays about: "cryo"

Showing result 1 - 5 of 37 essays containing the word cryo.

  1. 1. Exploring alternative surfactants to replace PEG within lipid nanoparticles

    University essay from Lunds universitet/Livsmedelsteknik och nutrition (master); Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)

    Author : Elina Edidovica; [2024]
    Keywords : Lipid nanoparticle; polyethelyne glycol PEG ; alpha-tomatine; N-hexadecyl beta d-maltoside HDM ; DOPC; DPPC; cholesterol; film hydration; stability study; DLS; Cryo-TEM; SAXS; pharmaceutical formulation; Chemistry;

    Abstract : Over the past decade an increasing amount of studies has demonstrated that PEGylation not only precipitates reductions in drug delivery efficiency but also triggers the immune system to produce antibodies, specifically anti-PEG antibodies. The objective of my thesis is to explore alternative surfactants that could viably replace PEG within lipid nanoparticles. READ MORE

  3. 2. Characterization of Mitilysin Pores by Cryo-electron Microscopy

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Vladimir Novakovic; [2023]
    Keywords : Mitilysin; Mly; Structural Biology; Cryo-EM; pore; toxin; pore forming proteins; pore forming toxins; PFP; PFT;

    Abstract : Pore forming toxins (PFTs) are a large group of proteins found mainly in bacteria with some exceptions found in animals. They bind and form pores in their target membranes and form pores, which leads to cell death. Among these are cholesterol-dependent cytolysins (CDC), which require the presence of cholesterol to bind target membranes. READ MORE

  4. 3. A Novel Method for Finding Overlap Depth : Development of ArtiaX, a ChimeraX plugin

    University essay from Linköpings universitet/Tillämpad matematik; Linköpings universitet/Tekniska fakulteten

    Author : Gunnar Arctaedius; [2023]
    Keywords : Cryo-electron tomography; image processing; surface visualization; overlap measurement; normal projection method; Kryoelektrontomografi; bildbehandling; ytvisualisering; överlapsmätning; normalprojektionsmetoden;

    Abstract : Visualization and image filtering are important parts of cryo-electron tomography analysis. ArtiaX, a plugin developed for UCSF ChimeraX, has been extended to improve the functionality of these two parts. For the visualization, a method of moving 3D surfaces to remove overlap between them has been developed and implemented. READ MORE

  5. 4. Towards time-resolved cryo-EM of SARS-CoV-2 replication-transcription complex and Staphylococcus aureus DNA gyrase

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Anna Králová; [2023]
    Keywords : time-resolved cryo-EM; DNA gyrase; SARS-CoV-2; replication-transcription complex; protein purification;

    Abstract : Time-resolved cryo-EM has already provided ground-breaking discoveries in various fields, including structural biology, biochemistry, and drug development. Compared to traditional structural biology methods where mostly stabilized conformations are reconstructed, the main advantage of time-resolved cryo-EM is its ability to capture dynamic processes in biological samples at near-atomic resolution, which allows for studying biological structures as they change and interact in real-time. READ MORE

  6. 5. Approaches to Structural Characterization of a Heteromeric GABA(A)R

    University essay from KTH/Tillämpad fysik

    Author : Alexander Stevens; [2023]
    Keywords : Applied Physics; Biophysics; Molecular Biophysics; GABA A R; LGIC; pLGIC; Tillämpad Fysik; Biofysik; Molekylär Biofysik; GABA A R; LGIC; pLGIC;

    Abstract : Structural biology has become an important part of researching various diseases and drug development. In this thesis, I provide details on how I worked with approaches to structural characterization of a heteromeric GABA(A)R. READ MORE