Essays about: "downstream purification"

Showing result 1 - 5 of 20 essays containing the words downstream purification.

  1. 1. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Keywords : Plasmid purification; pDNA; large-scale chromatography;

    Abstract : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. READ MORE

  3. 2. Geobacillus 7349 – A new potential biosurfactant producing microorganism

    University essay from Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Author : Zhuoying Deng; [2023]
    Keywords : biosurfactant; rhamnolipids; HPLC; HPAEC-PAD; bacteria growth; downstream purification; liquid-liquid extraction; biotechnology; Chemistry;

    Abstract : The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. READ MORE

  4. 3. The Examination of Alternative Methods for Sperm Cell Lysis and Purification of Sexual Assault Samples

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Emilie Boson; [2023]
    Keywords : Differential lysis; Differential extraction; Sexual assault samples; Sperm cells; Forensic; DNA recovery; DNA profiling; DNA purification; Applied microbiology; Technology and Engineering; Biology and Life Sciences;

    Abstract : Forensic sexual assault samples typically contain a mixture of a small quantity of sperm cells and a high quantity of epithelial cells. This relationship complicates the generation of a DNA profile, which prompts the need for differential lysis and extraction of the DNA. READ MORE

  5. 4. A high-throughput method for screening of protein binding behavior of multimodal anionic exchange ligands

    University essay from Uppsala universitet/Analytisk farmaceutisk kemi

    Author : Ani Avedis; [2021]
    Keywords : Multimodal chromatography; multimodal anionic exchange ligands; product development; purification process; biological drugs; resins;

    Abstract : The biopharmaceutical industry is constantly developing biological drugs, resulting in increased levels of product related impurities having similar characteristics as the target. The aim of the ligand project was to address future challenging purifications by developing new ligands for future resins for the biopharmaceutical industry. READ MORE

  6. 5. Advanced control of chromatography systems for automated analysis of monoclonal antibodies

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Jerry Guan; [2021]
    Keywords : Chemical engineering; Kemiteknik; Chromatography; Analysis; HPLC; Python; Automatic process control; Monoclonal Antibodies; Antibody purification; Technology and Engineering;

    Abstract : Nedströmsprocesser för rening av biologiska läkemedel som monoklona antikroppar rör sig från batch-processer till kontinuerliga, och vidare till helt automatiska, kontinuerliga nedströmsprocsser. En del av visionen för automatiska reningsprocesser är möjligheten att automatiskt analysera från olika steg av reningen. READ MORE