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Showing result 1 - 5 of 6 essays matching the above criteria.

  1. 1. Gene expression editing in myeloma cell lines using CRISPR/Cas9 technique

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Wilma Wadman; [2023]
    Keywords : BMPR1A; BMPR2; bone morphogenetic protein; gene knockout; multiple myeloma;

    Abstract : Multiple myeloma, or myeloma, is a bone marrow cancer which characterizes by uncontrolled proliferation of mutant plasma cells. It is a disease that claims many lives every year, mostly due to the absence of curative treatment. Finding a suitable treatment is therefor of great importance. READ MORE

  3. 2. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elnaz Samadian Zad; [2023]
    Keywords : adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Abstract : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. READ MORE

  4. 3. Production and validation of anti-HCV antibodies for viral neutralization

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Megan Olga García Mark; [2020]
    Keywords : Cloning; antibody; infection; HCV; IgG production; molecular biology; cell factory; plasmid design; immunology;

    Abstract : Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. READ MORE

  5. 4. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Alexander Westlund; [2019]
    Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

  6. 5. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    University essay from Linköpings universitet/Teknisk biologi

    Author : Malin Strannermyr; [2018]
    Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE