Essays about: "DNA sequence design"

Showing result 1 - 5 of 6 essays containing the words DNA sequence design.

  1. 1. Production and validation of anti-HCV antibodies for viral neutralization

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Megan Olga García Mark; [2020]
    Keywords : Cloning; antibody; infection; HCV; IgG production; molecular biology; cell factory; plasmid design; immunology;

    Abstract : Hepatitis-C Virus (HCV) remains the leading cause of liver transplant in the US and the UK, and the World Health Organization (WHO) estimates that 71 million people are infected worldwide. A vaccine would drastically impact the healthcare-associated burdens that HCV causes globally. READ MORE

  2. 2. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    University essay from Linköpings universitet/Teknisk biologi

    Author : Malin Strannermyr; [2018]
    Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE

  3. 3. Structural elucidation of mRNA(Sirt1)-microRNA 34a complex

    University essay from KTH/Skolan för teknikvetenskaplig kommunikation och lärande (ECE)

    Author : Mona Farshchian; [2015]
    Keywords : mRNA Sirt1 ; miR-34a; in vitro transcription; gel electrophoresis; CD spectroscopy; NMR; cancer regulation via p53; HPLC; mRNA Sirt1 ; miR-34a; in vitro transkription; gel elektroforesis; CD spektroskopi; NMR; cancer regulering via p53; HPLC;

    Abstract : The aim of this thesis is to understand RNA-RNA interactions steering cellular functions, as in the case of this thesis the structure of mRNA(Sirt1) in complex with microRNA-34a (miR-34a). MiR-34a regulates the cancer protein p53 via Sirt1 modulation. READ MORE

  4. 4. Nanostructures on a Vector : Enzymatic Oligo Production for DNA Nanotechnology

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologiLinköpings universitet/Tekniska högskolan

    Author : Camilla Sandén; [2012]
    Keywords : DNA origami; Rolling Circle Amplification; Phage; Scaffold; Oligonucleotides;

    Abstract : The technique of DNA origami utilizes the specific and limited bonding properties of DNA to fold single stranded DNA sequences of various lengths to form a predesigned structure. One longer sequence is used as a scaffold and numerous shorter sequences called staples, which are all complementary to the scaffold sequence, are used to fold the scaffold into intricate shapes. READ MORE

  5. 5. Development of novel multiplexed systems for in situ PLA

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : John Broberg; [2011]
    Keywords : Proximity ligation assay; PLA; Multiplex; Immunoassay; Rolling circle amplification; RCA; in situ; DNA sequence design;

    Abstract : The in situ proximity ligation assay (in situ PLA) is an immunoassay that enables directvisualisation of single protein targets or protein interactions in cell or tissue samples. This project revolves around designing and introducing several novel multiplexable components tobe used in conjunction with Olink Bioscience's Duolink product line. READ MORE