Essays about: "Gel filtration chromatography"

Showing result 1 - 5 of 8 essays containing the words Gel filtration chromatography.

  1. 1. Imaging of the Cytosolic Antibody Receptor TRIM21

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Þórunn Stefánsdóttir; [2022]
    Keywords : TRIM21; ProteinStructure; Antibody; cryo-EM; Gel-Filtration SizeExclusion Chromatography ; Immunology; ProteinPurification; SDS-PAGE; TRIM21; Proteinstruktur; Antikroppar; cryo-EM; Gel-filtreringsKromatografi; Immunologi; ProteinRening; SDS-PAGE;

    Abstract : TRIM21 is a cytosolic ubiquitin ligase and an antibody receptor that providesa last line of defense against invading pathogens. By utilizing the diversity ofantibody repertoire to identify pathogens, TRIM21 serves as a link betweenintrinsic cellular defense and adaptive immunity. READ MORE

  2. 2. Implementation of the mille-feuille nanofilter paper in the virus removal filtration of IgY purified from chicken egg yolk

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Michelle Dahlman; [2020]
    Keywords : ;

    Abstract : The purpose of this thesis was to test the mille-feuille nanofilter paper for a virus removal filtration from a potential protein-based nutraceutical. Therefore, chicken IgY was purified from egg yolk, with PEG precipitation for crude fractionation followed by thiophilic adsorption chromatography. READ MORE

  3. 3. Production of gangliosidebiosynthetic membrane enzymes for biochemical and functionalstudies : Expression, purification and crystallizationoptimization of Thermococcus onnurineus Dolicho l-phosphate mannose synthase, Homosapiens and Branchiostoma floridae Glucosylceramide synthase

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Ellinor Lindholm; [2018]
    Keywords : glycoconjugates; glycolipids; integral membrane proteins; gangliosides; glucosylceramide synthase; dolicholphosphate mannose synthase; cancer; neuroblastoma; congenital disorders of glycosylation; glykokonjukat; glykolipider; membranprotein; gangliosider; glukosylceramidsyntas; dolicholfosfat mannossyntas; cancer; neuroblastom; medfdd glykosyleringssjukdom;

    Abstract : Glycolipids play important roles in the biology of prokaryotes and eukaryotes, including humans, and although theyare found on the cell-membrane surface of all eukaryotic cells, not much is known about their biosynthesis. The aim ofthis project was to characterize two enzymes: glucosylceramide synthase (GCS) which is involved in the biosynthesisof glycolipids such as gangliosides that are abundant in the membranes of nerve cells; and dolicholphosphate mannosesynthase (DPMS), involved in the synthesis precursor for protein glycosylation. READ MORE

  4. 4. Characterisation of free and conjugated protease inhibitors from Solanum tuberosum

    University essay from Uppsala universitet/Institutionen för kemi - BMC

    Author : Kristoffer Lundmark; [2017]
    Keywords : enzyme; trypsin; chymotrypsin; pancrease protease; serine protease inhibitors; SPI; potato tubers; potato; Solanum tuberosum; immobilized; aluminium oxide membranes; inhibitor-enzyme interaction;

    Abstract : The main purpose of the master thesis project is to investigate the influence of selected serine protease inhibitors (SPI) on the catalytic action of the serine proteases chymotrypsin and trypsin, in a conjugated and non-conjugated state. The inhibitors included for this study were extracted from Solanum tuberosum, i.e.common potato. READ MORE

  5. 5. Biochemical analysis of type II Metacaspase ( mcII - Pa )

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Mahmudur Rahman; [2010]
    Keywords : Metacaspase; appoptosis; Norway spruce; caspase; biochemical; plant cell death;

    Abstract : Programmed Cell Death (PCD) is a physiological process of cell death to remove unwanted cells and damaged cells. This physiological process is a cascade of biochemical reactions triggered and completed by Caspase, a cysteine protease, in animals. After discovering PCD in plants scientists tried to find caspase or similar enzyme in plants. READ MORE