Essays about: "Fusion Protein"

Showing result 16 - 20 of 40 essays containing the words Fusion Protein.

  1. 16. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Alexander Westlund; [2019]
    Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

  3. 17. The synthesis and analysis of a bombesin analogue for radiotherapy of prostate cancer

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Ábel Nagy; [2019]
    Keywords : Prostate cancer; radionuclide therapy; bombesin; gastrin-releasing peptide receptor; albumin binding domain; SPPS; RM26 analogue;

    Abstract : Targeted radionuclide therapy is becoming a widely used cancer treatment strategy. By radiolabeling receptor-specific peptides, cancer cells overexpressing the receptor can be selectively targeted, and the cytotoxic radionuclide can be delivered to the target cell or tissue for therapeutic or diagnostic purposes. READ MORE

  4. 18. Fluorescent fusion proteins as probes to characterize tau fibril polymorphism

    University essay from Linköpings universitet/Kemi

    Author : Max Lindberg; [2019]
    Keywords : Alzheimer’s disease AD ; amyloids; FRET; tau; MAPT; fibrillation kinetics; seeding; fusion protein; EGFP; ECFP; EYFP; ThT; X-34; BTD14;

    Abstract : Alzheimer's disease (AD) is a large and growing problem and while we today lack a full understanding of this disease, we know that the protein tau and the amyloid fibrils it forms play a central role in its development. We also know that these fibrils can have different morphologies in different diseases and that fibrils produced in vitro not necessarily adopt any of the morphologies found in patients. READ MORE

  5. 19. Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Miguel Ángel Postigo Peláez; [2019]
    Keywords : NLRP3; Inflammation; Fusion gene; PCR; Fusion protein;

    Abstract : Inflammasomes are a group of protein complex that regulate inflammation throughcomplex signal transduction, although their specific mechanisms and structures have notbeen fully described. As the protein that kickstarts assembly of a type of inflammasome,NLRP3 is a key regulator of inflammation and may play a relevant role in the developmentof inflammatory diseases. READ MORE

  6. 20. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    University essay from Linköpings universitet/Teknisk biologi

    Author : Malin Strannermyr; [2018]
    Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE