Essays about: "Oligonucleotide"

Showing result 1 - 5 of 17 essays containing the word Oligonucleotide.

  1. 1. A novel assay for the detection of Hepatitis C virus in blood plasma, using padlock probes and rolling circle amplification

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Andréa Karlsson; [2023]
    Keywords : ;

    Abstract : Hepatitis C viral infection is a globally widespread blood-borne disease affecting the liver, causing cirrhosis and hepatocellular carcinoma. This type of liver cancer is mainly caused by chronic Hepatitis C and Hepatitis B. Specific detection with the following correct treatment is crucial to reduce the overall burden of the disease. READ MORE

  3. 2. Signal amplification in a microfluidic immunoassay system via Binding Oligo Ladder Detection : Applying the Exazym® signal amplification to the Gyrolab® platform

    University essay from Uppsala universitet/Institutionen för farmaci

    Author : Daniel Wiman; [2023]
    Keywords : immunoassay; ultra-sensitive; signal amplification; Gyrolab; Exazym; high sensitivity; microfluidics; BOLD amplification; nucleic acid polymerisation;

    Abstract : Immunoassays are analytical methods that use the highly specific binding of antibodies in order to detect and quantify an analyte. The technique has become a staple in modern biopharmaceutical research and diagnostics, however the measurement of biomarkers like dysregulated cytokines require ultra-sensitive immunoassays that can detect molecules at sub pg/mL concentrations. READ MORE

  4. 3. Non-coding RNAs as therapeutic agents : The future of therapy

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Hederoth Gustaf; Lyngå Olivia; Rudqvist Olivia; Jeppsson Rebecka; Hogolof Saida; Svanberg Sara; Kauppinen Veera; [2022]
    Keywords : Non-coding RNA; Oligonucleotide therapeutics;

    Abstract : Non-coding RNA (ncRNA) therapeutics are based on short oligonucleotides, both naturally occurring and artificial, which target RNA in a site-specific way to modulate gene expression. As of today, 12 synthetically produced ncRNA-based drugs are available on the market in the US and Europe, and there is a possibility of more to be approved in the near future. READ MORE

  5. 4. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

    University essay from Karlstads universitet

    Author : Lewis Frazer; [2021]
    Keywords : Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

    Abstract : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. READ MORE

  6. 5. The dependence of chromatographic conditions for separation of oligonucleotides in different AEX-HPLC columns

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Julia Nylander; [2020]
    Keywords : ion exchange chromatography; oligonucleotides; anion exchange column; pH; HPLC; Jonbyteskromatografi; oligonukleotider; anjonbytarkolonn; pH; HPLC;

    Abstract : Oligonucleotides (ONs) are widely used in different applications in life science, forensic, in i.e. family tree DNA test for humans and in diagnostic applications in several fields. READ MORE